Comparative Study of Four Techniques for Serodiagnosis of Parainfluenza Virus Type 1 in Pigs
نویسندگان
چکیده
Sera obtained from 777 pigs were tested for the presence of Sendai virusimmunospecific antibodies by the agar gel immunodiffussion (AGID), counterimmunoelectrophoresis (CIE), haemagglutination inhibition (HI) and serum neutralisation (SN) tests. As many as 598 sera contained Sendai virus antibodies as shown by the SN test, 552 sera were HI positive, 536 sera were CIE positive, and only 444 ones were AGID positive. When the SN test was applied as the reference one, the sensitivity and specificity of the HI, CIE, and AGID tests were found to be 90.96% and 95.53%, 88.29% and 95.53%, 67.75% and 77.65%, respectively. The analytical accuracy of the HI and CIE tests relatively to the SN test was similar. The good consistency between the HI and CIE tests (κ=0.98) suggests that the two methods are interchangeable.
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